Effect of Leptin on the Expression of Chemokine Genes in THP-1 Cells / 영남의대학술지
Yeungnam University Journal of Medicine
;
: 129-141, 2003.
Article
in Korean
| WPRIM
| ID: wpr-143808
ABSTRACT
BACKGROUND:
Leptin is a 16-KDa non-glycosylated peptide hormone synthesized almost exclusively by adipocytes. The well-known function of leptin is regulation of food intake and energy expenditure. Leptin also plays a regulatory role in immune and inflammatory process including cytokine production. The purpose of this study was to investigate the effect of leptin on the expression of several chemokine genes(RANTES, IL-8, MCP-1, IP-10, Mig, MIP-1alpha, MIP-1beta, and GRO-alpha) in THP-1 cells. MATERIALS ANDMETHODS:
Total RNA of THP-1 cells were prepared by Trizol method, and then stimulated with the leptin(250 ng/microliter) or LPS(100 ng/microliter). We examined the expression patterns of various chemokine mRNAs in THP-1 cell lines by RT-PCR and Northern blot.RESULTS:
Leptin did not induce the expression of chemokine mRNAs in THP-1 cells. The expression patterns of RANTES, IL-8, MCP-1, IP-10, and Mig mRNAs in THP-1 cells stimulated with leptin and LPS simultaneously was almost same to the patterns of LPS alone-induced chemokine mRNAs. RANTES mRNA expression was independent on the concentrations of leptin. Although leptin did not have strong effect on the expression of RANTES, IL-8, MCP-1, IP-10, Mig, MIP-1alpha, MIP-1beta, and GRO-alpha mRNAs in THP-1 cells, leptin could induce the expression of long isoform of leptin receptor(OB-RL) mRNA, and its expression was elevated in simultaneous stimulation of leptin and LPS.CONCLUSION:
These data suggest that leptin is able to induce OB-RL in THP-1 cells, however, leptin has little effect on the expression of pro-inflammatory chemokine genes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA
/
RNA, Messenger
/
Cell Line
/
Blotting, Northern
/
Interleukin-8
/
Adipocytes
/
Chemokine CCL5
/
Leptin
/
Eating
/
Energy Metabolism
Language:
Korean
Journal:
Yeungnam University Journal of Medicine
Year:
2003
Type:
Article
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