Effect of Dexamethasone on the Production of Nitric Oxide in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
;
: 1254-1258, 2009.
Article
in Korean
| WPRIM
| ID: wpr-144207
ABSTRACT
PURPOSE:
To investigate the effects of dexamethasone (DEX) on the production of nitric oxide (NO) and its enzymatic synthetic pathway in cultured human trabecular meshwork (HTM) cells.METHODS:
Primarily cultured HTM cells were exposed to 0, 10, 100, 1000 nM of DEX for 3 days. In addition, 100 micrometer sepiapterin, 100 micrometer ascorbic acid, and 10 micrometer methotrexate were co-exposed to DEX. The cellular survival and nitrite production rates were assessed by MTT assay and Griess assay, respectively.RESULTS:
DEX did not significantly affect the survival of cultured HTM cells. DEX decreased the NO production in a dose-dependent manner. With co-exposure of DEX, ascorbic acid nullified the DEX-induced decrease of NO production. Sepiapterin and methotrexate did not affect DEX-induced decrease of NO production.CONCLUSIONS:
DEX decreased NO production in HTM cells and the de novo pathway of tetrahydrobiopterin may be involved. This decrease may raise intraocular pressure by decreasing trabecular outflow.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pterins
/
Ascorbic Acid
/
Trabecular Meshwork
/
Biopterin
/
Dexamethasone
/
Methotrexate
/
Intraocular Pressure
/
Nitric Oxide
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2009
Type:
Article
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