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Effect of Dexamethasone on the Production of Nitric Oxide in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society ; : 1254-1258, 2009.
Article in Korean | WPRIM | ID: wpr-144207
ABSTRACT

PURPOSE:

To investigate the effects of dexamethasone (DEX) on the production of nitric oxide (NO) and its enzymatic synthetic pathway in cultured human trabecular meshwork (HTM) cells.

METHODS:

Primarily cultured HTM cells were exposed to 0, 10, 100, 1000 nM of DEX for 3 days. In addition, 100 micrometer sepiapterin, 100 micrometer ascorbic acid, and 10 micrometer methotrexate were co-exposed to DEX. The cellular survival and nitrite production rates were assessed by MTT assay and Griess assay, respectively.

RESULTS:

DEX did not significantly affect the survival of cultured HTM cells. DEX decreased the NO production in a dose-dependent manner. With co-exposure of DEX, ascorbic acid nullified the DEX-induced decrease of NO production. Sepiapterin and methotrexate did not affect DEX-induced decrease of NO production.

CONCLUSIONS:

DEX decreased NO production in HTM cells and the de novo pathway of tetrahydrobiopterin may be involved. This decrease may raise intraocular pressure by decreasing trabecular outflow.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pterins / Ascorbic Acid / Trabecular Meshwork / Biopterin / Dexamethasone / Methotrexate / Intraocular Pressure / Nitric Oxide Limits: Humans Language: Korean Journal: Journal of the Korean Ophthalmological Society Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pterins / Ascorbic Acid / Trabecular Meshwork / Biopterin / Dexamethasone / Methotrexate / Intraocular Pressure / Nitric Oxide Limits: Humans Language: Korean Journal: Journal of the Korean Ophthalmological Society Year: 2009 Type: Article