Production of TGF-beta1 as a Mechanism for Defective Antigen-presenting Cell Function of Macrophages Generated in vitro with M-CSF
Immune Network
;
: 27-33, 2009.
Article
in English
| WPRIM
| ID: wpr-144443
ABSTRACT
BACKGROUND:
Macrophages generated in vitro using macrophage-colony stimulating factor (M-CSF) and interleukin (IL)-6 from bone marrow cells (BM-Mp) are defective in antigen presenting cell (APC) function as shown by their ability to induce the proliferation of anti-CD3 mAb-primed syngeneic T cells. However, they do express major histocompatibility (MHC) class I and II molecules, accessory molecules and intracellular adhesion molecules. Here we demonstrate that the defective APC function of macrophages is mainly due to production of TGF-beta1 by BM-Mp.METHODS:
Microarray analysis showed that TGF-beta1 was highly expressed in BM-Mp, compared to a macrophage cell line, B6D, which exerted efficient APC function. Production of TGF-beta1 by BM-Mp was confirmed by neutralization experiments of TGF-beta1 as well as by real time-polymerase chain reaction (PCR).RESULTS:
Addition of anti-TGF-beta1 monoclonal antibody to cultures of BM-Mp and anti-CD3 mAb-primed syngeneic T cells efficiently induced the proliferation of syngeneic T cells. Conversely, the APC function of B6D cells was almost completely suppressed by addition of TGF-beta1. Quantitative real time-PCR analysis also confirmed the enhanced expression of TGF-beta1 in BM-Mp.CONCLUSION:
The defective APC function of macrophages generated in vitro with M-CSF and IL-6 was mainly due to the production of TGF-beta1 by macrophages.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bone Marrow Cells
/
T-Lymphocytes
/
Cell Line
/
Macrophage Colony-Stimulating Factor
/
Interleukins
/
Interleukin-6
/
Microarray Analysis
/
Transforming Growth Factor beta1
/
Histocompatibility
/
Macrophages
Language:
English
Journal:
Immune Network
Year:
2009
Type:
Article
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