Your browser doesn't support javascript.
loading
D60-sensitive tyrosine phosphorylation is involved in Fas-mediated phospholipase D activation
Experimental & Molecular Medicine ; : 303-309, 2001.
Article in English | WPRIM | ID: wpr-144612
ABSTRACT
Both Fas and PMA can activate phospholipase D via activation of protein kinase Cbeta in A20 cells. Phospholipase D activity was increased 4 fold in the presence of Fas and 2.5 fold in the presence of PMA. The possible involvement of tyrosine phosphorylation in Fas-induced activation of phospholipase D was investigated. In five minute after Fas cross-linking, there was a prominent increase in tyrosine phosphorylated proteins, and it was completely inhibited by D609, a specific inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC). A tyrosine kinase inhibitor, genistein, can partially inhibit Fas-induced phospholipase D activation. There were no effects of genistein on Fas-induced activation of PC-PLC and protein kinase C. These results strongly indicate that tyrosine phosphorylation may in part account for the increase in phospholipase D activity by Fas cross-linking and D609 can block not only PC-PLC activity but also tyrosine phosphorylation involved in Fas-induced phospholipase D activation.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Type C Phospholipases / Phospholipase D / Phosphorylation / Phosphorylcholine / Solubility / Thiones / Tyrosine / Bridged-Ring Compounds / Tumor Cells, Cultured / Water Type of study: Diagnostic study Limits: Animals Language: English Journal: Experimental & Molecular Medicine Year: 2001 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Type C Phospholipases / Phospholipase D / Phosphorylation / Phosphorylcholine / Solubility / Thiones / Tyrosine / Bridged-Ring Compounds / Tumor Cells, Cultured / Water Type of study: Diagnostic study Limits: Animals Language: English Journal: Experimental & Molecular Medicine Year: 2001 Type: Article