Diagnosis of canine brucellosis using recombinant ribosomal protein L7/L12 / 대한수의학회지
Korean Journal of Veterinary Research
;
: 25-31, 2012.
Article
in Korean
| WPRIM
| ID: wpr-145340
ABSTRACT
Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Placenta
/
Ribosomal Proteins
/
Brucella
/
Brucellosis
/
Enzyme-Linked Immunosorbent Assay
/
Sensitivity and Specificity
/
Clone Cells
/
Veterinarians
/
Aborted Fetus
/
Brucella canis
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Veterinary Research
Year:
2012
Type:
Article
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