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The Resveratrol-induced Relaxation of Cholecystokinin Octapeptide- or KCl-induced Tension in Male Guinea Pig Gallbladder Strips Is Mediated Through L-type Ca2+ Channels
Journal of Neurogastroenterology and Motility ; : 62-68, 2015.
Article in English | WPRIM | ID: wpr-14535
ABSTRACT
BACKGROUND/

AIMS:

Resveratrol (3,5,4'-trihydroxystilbene) is a polyphenolic compound (stilbene) and a phytoalexin. The purpose of this study was to determine the mechanism which mediated the resveratrol-induced relaxation of cholecystokinin octapeptide- or KCl-induced tension in male guinea pig gallbladder strips.

METHODS:

Gallbladder strips were prepared and suspended in in vitro chambers filled with Krebs-Henseleit solution. The strips were attached to force displacement transducers, and the changes in tension were recorded on a polygraph. All reagents were added directly into the chambers.

RESULTS:

To determine if intracellular Ca2+ release mediated the resveratrol-induced relaxation of cholecystokinin octapeptide-induced tension, 2-aminoethoxydiphenylborane (2-APB) was used. 2-APB significantly (P < 0.01) decreased the amount of RSVL-induced relaxation. To determine if protein kinase A (PKA) mediated the resveratrol-induced relaxation, PKA inhibitor 14-22 amide myristolated (PKA-IM) was used. PKA-IM had no effect on resveratrol-induced relaxation. Neither KT5823, N(G)-methyl-L-arginine acetate salt, a nitric oxide synthase inhibitor, nor fulvestrant had a significant effect on the amount of resveratrol-induced relaxation. Genistein, a protein tyrosine kinase inhibitor, significantly (P < 0.01) increased the RSVL-induced relaxation. To determine if protein kinase C mediated the RSVL-induced relaxation, the protein kinase C inhibitors bisindolymaleimide IV and chelerythrine Cl- were used together, and a significant (P < 0.05) increase in resveratrol-induced relaxation was observed. The pretreatment of the strips with resveratrol significantly (P < 0.001) decreased the amount of KCl- and cholecystokinin octapep-tide-induced tension.

CONCLUSIONS:

Resveratrol-induced relaxation is mediated by its effects on L-type Ca2+ channels and intracellular Ca2+ release.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Relaxation / Transducers / Protein Kinase C / Protein-Tyrosine Kinases / Cholecystokinin / Calcium Channels / Cyclic AMP-Dependent Protein Kinases / Nitric Oxide Synthase / Genistein / Gallbladder Limits: Animals / Humans / Male Language: English Journal: Journal of Neurogastroenterology and Motility Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Relaxation / Transducers / Protein Kinase C / Protein-Tyrosine Kinases / Cholecystokinin / Calcium Channels / Cyclic AMP-Dependent Protein Kinases / Nitric Oxide Synthase / Genistein / Gallbladder Limits: Animals / Humans / Male Language: English Journal: Journal of Neurogastroenterology and Motility Year: 2015 Type: Article