Regulatory Role of Autophagy in Globular Adiponectin-Induced Apoptosis in Cancer Cells
Biomolecules & Therapeutics
;
: 384-389, 2014.
Article
in English
| WPRIM
| ID: wpr-145969
ABSTRACT
Adiponectin, an adipokine predominantly secreted from adipose tissue, exhibits diverse biological responses, including metabolism of glucose and lipid, and apoptosis in cancer cells. Recently, adiponectin has been shown to modulate autophagy as well. While emerging evidence has demonstrated that autophagy plays a role in the modulation of proliferation and apoptosis of cancer cells, the role of autophagy in apoptosis of cancer cell caused by adiponectin has not been explored. In the present study, we demonstrated that globular adiponectin (gAcrp) induces both apoptosis and autophagy in human hepatoma cell line (HepG2 cells) and breast cancer cells (MCF-7), as evidenced by increase in caspase-3 activity, Bax, microtubule-associated protein light chain 3-II (LC3 II) protein levels, and autophagosome formation. Interestingly, gene silencing of LC3B, an autophagy marker, significantly enhanced gAcrp-induced apoptosis in both HepG2 and MCF-7 cell lines, whereas induction of autophagy by rapamycin, an mTOR inhibitor, significantly prevented gAcrp-induced apoptosis in hepatoma cells HepG2. Furthermore, modulation of autophagy produced similar effects on gAcrp-induced Bax expression in HepG2 cells. These results implicate that induction of autophagy plays a regulatory role in adiponectin-induced apoptosis of cancer cells, and thus inhibition of autophagy would be a novel promising target to enhance the efficiency of cancer cell apoptosis by adiponectin.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Autophagy
/
Breast Neoplasms
/
Cell Line
/
Adipose Tissue
/
Apoptosis
/
Carcinoma, Hepatocellular
/
Sirolimus
/
Gene Silencing
/
Adiponectin
/
Caspase 3
Limits:
Humans
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2014
Type:
Article
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