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Granulocyte Colony Stimulating Factor Attenuates Hyperoxia-Induced Lung Injury by Down-Modulating Inflammatory Responses in Neonatal Rats
Yonsei Medical Journal ; : 65-73, 2011.
Article in English | WPRIM | ID: wpr-146144
ABSTRACT

PURPOSE:

Granulocyte colony stimulating factor (G-CSF) has been known to increase neutrophil production and have anti-inflammatory properties, but the effect of G-CSF on pulmonary system is in controversy. We investigated whether G-CSF treatment could attenuate hyperoxia-induced lung injury, and whether this protective effect is mediated by the down-modulation of inflammatory responses in a neonatal rat model. MATERIALS AND

METHODS:

Newborn Sprague-Dawley rats (Orient Co., Seoul, Korea) were subjected to 14 days of hyperoxia (90% oxygen) beginning within 10 h after birth. G-CSF (20 microg/kg) was administered intraperitoneally on the fourth, fifth, and sixth postnatal days.

RESULTS:

This treatment significantly improved hyperoxia-induced reduction in body weight gain and lung pathology such as increased mean linear intercept, mean alveolar volume, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling positive cells. Hyperoxia-induced activation of nicotinamide adenine dinucleotide phosphate oxidase, which is responsible for superoxide anion production, as evidenced by upregulation and membrane translocation of p67phox was significantly attenuated after G-CSF treatment, as were inflammatory responses such as increased myeloperoxidase activity and mRNA expression of transforming growth factor-beta. However, the attenuation of other proinflammatory cytokines such as tumor necrosis factor-alpha and interleukin-6 was not significant.

CONCLUSION:

In sum, G-CSF treatment significantly attenuated hyperoxia-induced lung injury by down-modulating the inflammatory responses in neonatal rats.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Weight Gain / Random Allocation / Blotting, Western / Granulocyte Colony-Stimulating Factor / Transforming Growth Factor beta / Interleukin-6 / Tumor Necrosis Factor-alpha / Rats, Sprague-Dawley / Peroxidase / Hyperoxia Type of study: Prognostic study Limits: Animals / Pregnancy Language: English Journal: Yonsei Medical Journal Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Weight Gain / Random Allocation / Blotting, Western / Granulocyte Colony-Stimulating Factor / Transforming Growth Factor beta / Interleukin-6 / Tumor Necrosis Factor-alpha / Rats, Sprague-Dawley / Peroxidase / Hyperoxia Type of study: Prognostic study Limits: Animals / Pregnancy Language: English Journal: Yonsei Medical Journal Year: 2011 Type: Article