Granulocyte Colony Stimulating Factor Attenuates Hyperoxia-Induced Lung Injury by Down-Modulating Inflammatory Responses in Neonatal Rats
Yonsei Medical Journal
;
: 65-73, 2011.
Article
in English
| WPRIM
| ID: wpr-146144
ABSTRACT
PURPOSE:
Granulocyte colony stimulating factor (G-CSF) has been known to increase neutrophil production and have anti-inflammatory properties, but the effect of G-CSF on pulmonary system is in controversy. We investigated whether G-CSF treatment could attenuate hyperoxia-induced lung injury, and whether this protective effect is mediated by the down-modulation of inflammatory responses in a neonatal rat model. MATERIALS ANDMETHODS:
Newborn Sprague-Dawley rats (Orient Co., Seoul, Korea) were subjected to 14 days of hyperoxia (90% oxygen) beginning within 10 h after birth. G-CSF (20 microg/kg) was administered intraperitoneally on the fourth, fifth, and sixth postnatal days.RESULTS:
This treatment significantly improved hyperoxia-induced reduction in body weight gain and lung pathology such as increased mean linear intercept, mean alveolar volume, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling positive cells. Hyperoxia-induced activation of nicotinamide adenine dinucleotide phosphate oxidase, which is responsible for superoxide anion production, as evidenced by upregulation and membrane translocation of p67phox was significantly attenuated after G-CSF treatment, as were inflammatory responses such as increased myeloperoxidase activity and mRNA expression of transforming growth factor-beta. However, the attenuation of other proinflammatory cytokines such as tumor necrosis factor-alpha and interleukin-6 was not significant.CONCLUSION:
In sum, G-CSF treatment significantly attenuated hyperoxia-induced lung injury by down-modulating the inflammatory responses in neonatal rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Weight Gain
/
Random Allocation
/
Blotting, Western
/
Granulocyte Colony-Stimulating Factor
/
Transforming Growth Factor beta
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Rats, Sprague-Dawley
/
Peroxidase
/
Hyperoxia
Type of study:
Prognostic study
Limits:
Animals
/
Pregnancy
Language:
English
Journal:
Yonsei Medical Journal
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS