Expression of Intracellular Single Chain Antibody Specific to Hepatitis B Virus X Protein
Immune Network
;
: 23-28, 2003.
Article
in Korean
| WPRIM
| ID: wpr-146213
ABSTRACT
BACKGROUND:
Intracellular antibody specific to hepatitis B virus X protein (HBx) might be useful for studying the role of HBx in hepatocellular carcinogenesis and HBV replication.METHODS:
With variable region genes for H7 monoclonal anti-HBx Ab, we constructed a vector for bacterial expression of single chain Ab (scFv) and a vector for eukaryotic cell expression of it. The expression of H7 scFv and its binding activity against HBx was examined by immunoblotting and immunofluorescence microscopy.RESULTS:
H7 scFv expressed in bacterial cells retained reactivity to HBx. We demonstrated its intracytoplasmic expression in CosM6 eukaryotic cells.CONCLUSION:
This is the first study showing the expression of intracellular anti-HBx Ab in eukaryotic cells. H7 scFv may be a good tool to study the function of HBx in HBV infection
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunoblotting
/
Hepatitis B virus
/
Eukaryotic Cells
/
Carcinogenesis
/
Hepatitis
/
Hepatitis B
/
Microscopy, Fluorescence
Language:
Korean
Journal:
Immune Network
Year:
2003
Type:
Article
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