Early Prediction of Hypoxic-Ischemic Brain Damage in Newbon Rats Using Proton Magnetic Resonance Spectroscopy and Neuroprotective Effect of EGb 761
Journal of the Korean Society of Neonatology
;
: 133-140, 2001.
Article
in Korean
| WPRIM
| ID: wpr-146419
ABSTRACT
PURPOSE:
This study was designed to evaluated the relationship between the morphological changes of hypoxic-ischemic injured brain and the magnetic resonance spectroscopic (MRS) findings, and the efficacy of Egb 761, a free radical scavenger, as a neuroprotective agent for hypoxic-ischemic brain injury.METHODS:
Seven-day old Sprague-Dawley rat pups were used. Right common carotid artery was ligated under halothene anesthesia. After a recovery period of 3 hours, they were divided into two groups for group 1, EGb 761 100 mg/kg (treatment group) and for group 2, normal saline (control group) were given intraperitoneally. Both groups were exposed to 8% oxygen at 37degrees C for 90 minutes. Sixty-four rat pups (30 control, 34 treatment group) were examined with localized 1H MRS on days 1 and 7 after the hypoxic insult. One day 14, the degree of brain injury was scored by morphological changes.RESULTS:
The 1H MRS obtained on day 1 after the hypoxic insult showed increased Lip/NAA and Lip/Cr ratios in the right cerebral hemisphere in comparison with those in the left hemisphere (P<0.01). The degree of morphological changes of the brain injury on day 14 correlated with both Lip/NAA and Lip/Cr ratios obtained on days! and 7 after the hypoxic insult [r=0.410, 0.457 on day 1, and r=0.749, 0.720 on day 7, respectively (P< 0.01)]. The therapeutic potential of EGb 761 for the hypoxic-ischemic brain seemed insignificant, as was evident from no differences between the control group and the EGb 761 treatment group in the Lip/NAA and Lip/Cr ratios as well as in morphological changes.CONCLUSION:
Early changes of Lip/NAA and Lip/Cr ratios in the 1H MRS seems to be related to the degree of morphological changes in the rat brain of hypoxic-ischemic injury. Therefore, the increased lipid content of brain observed by 1H MRS can be used as a marker to predict the degree of the hypoxic-ischemic injury. The neuroprotectiveeffect of EGb 761 in hypoxic-ischemic brain injury is insignificant.
Full text:
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Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Protons
/
Brain
/
Brain Injuries
/
Magnetic Resonance Spectroscopy
/
Rats, Sprague-Dawley
/
Carotid Artery, Common
/
Neuroprotective Agents
/
Cerebrum
/
Anesthesia
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Society of Neonatology
Year:
2001
Type:
Article
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