Development of a Tissue-Engineered Vascular Graft Using Autologous Bone Marrow-Derived Cells and Biodegradable Polymer Scaffold
Journal of the Korean Society for Vascular Surgery
;
: 124-131, 2003.
Article
in Korean
| WPRIM
| ID: wpr-146577
ABSTRACT
PURPOSE:
The objective of this study is to develop a tissue-engineered vascular graft using autologous bone marrow-derived cells (BMCs) and biodegradable polymer scaffold.METHOD:
Autologous canine BMCs were isolated from bone marrow aspirate and cultured. A tubular scaffold was fabricated by immersing polyglycolic acid (PGA) sheet in poly (glycolide-co-caprolactone) (PGCL) solution and wrapping it around a cylindrical mold. The expanded BMCs were seeded onto the PGA/PGCL tubular scaffold (internal diameter 7 mm, length 35 mm) and further cultured in vitro for 1 week. The graft was anastomosed to the abdominal artery in a canine model. One week after implantation, the retrieved graft was investigated by histological and immunohistochemical analyses.RESULT:
Cultured BMCs differentiated into endothelial-like and smooth muscle-like cells. The PGA tubular scaffold reinforced with PGCL was successfully implanted in an animal model without graft rupture. The vascular graft engineered with BMCs was occluded at 1 week after implantation due to thrombus formation. Histological and immunohistochemical analyses of the retrieved graft revealed that extracellular matrix proteins such as smooth muscle alpha-actin, smooth muscle myosin heavy chain and collagen were produced partially in the graft media.CoNCLUSION:
The tissue-engineered vascular graft developed in this study led to graft failure due to early occlusion. Nevertheless, it is confirmed that the PGA/PGCL scaffold has microstructures appropriate for cell proliferation and good mechanical properties. This result suggests the possibile application of this scaffold as a material for engineering of diseased vascular tissues.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polyglycolic Acid
/
Polymers
/
Arteries
/
Rupture
/
Thrombosis
/
Bone Marrow
/
Blood Vessel Prosthesis
/
Extracellular Matrix Proteins
/
Actins
/
Collagen
Language:
Korean
Journal:
Journal of the Korean Society for Vascular Surgery
Year:
2003
Type:
Article
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