Detection of Respiratory Viruses in Children by Multiplex Reverse Transcriptase PCR, Direct Immunofluorescence Assay, and Shell Vial Culture / 대한임상미생물학회지
Korean Journal of Clinical Microbiology
;
: 110-115, 2009.
Article
in Korean
| WPRIM
| ID: wpr-146801
ABSTRACT
BACKGROUND:
Direct immunofluorescence assay (DFA) and shell vial culture (SVC) have been used to diagnose respiratory viral infections. Recently a multiplex reverse transcriptase PCR (mRT-PCR) for 12 respiratory viruses has been introduced. We evaluated the diagnostic usefulness of these methods.METHODS:
Among 275 nasopharyngeal aspirates (NPAs) received from pediatric patients during the 3-month period from May through July, 2007, 122 samples were selected so as to include diverse viruses and varying numbers of DFA-positive cells for mRT-PCR. Also, the results of the 85 NPAs that had been analyzed by both DFA and SVC were reviewed retrospectively.RESULTS:
Detection rates for the seven major respiratory viruses, respiratory syncytial virus (RSV), influenza virus A and B, parainfluenza virus 1, 2, and 3, and adenovirus by DFA vs mRT-PCR were 32.0% and 55.7%, and by DFA vs SVC were 32.9% and 40.0%. A number of adenovirus detected by DFA vs mRT-PCR were 12 and 22, and by DFA vs SVC were 6 and 18. A number of RSV detected were 3 and 6, and 13 and 8, respectively.CONCLUSIONS:
mRT-PCR detected the respiratory viruses at the highest rate, followed by SVC and DFA in a decreasing order. However, DFA and multiplex PCR were more sensitive than SVC for RSV, while SVC was more sensitive than the other methods for adenovirus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Orthomyxoviridae
/
Respiratory Syncytial Viruses
/
Viruses
/
Adenoviridae
/
Fluorescent Antibody Technique
/
RNA-Directed DNA Polymerase
/
Paramyxoviridae Infections
/
Fluorescent Antibody Technique, Direct
/
Reverse Transcriptase Polymerase Chain Reaction
/
Multiplex Polymerase Chain Reaction
Type of study:
Diagnostic study
Limits:
Child
/
Humans
Language:
Korean
Journal:
Korean Journal of Clinical Microbiology
Year:
2009
Type:
Article
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