Reactive oxygen species and N-methyl-D-aspartate receptor-mediated central sensitization in hindlimb ischemia/reperfusion injury-induced neuropathic pain rats / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 186-194, 2009.
Article
in Korean
| WPRIM
| ID: wpr-146832
ABSTRACT
BACKGROUND:
Reactive oxygen species (ROS) contribute to development of neuropathic pain. A neuropathic pain syndrome was produced in rats following prolonged hindpaw ischemia/reperfusion injury, creating an animal model of complex regional pain syndrome-Type I (CRPS-I). This study was designed to evaluate the validity of this model for ROS and pain research. Herein we show superoxide produces N-methyl-D-aspartate (NMDA) mediated mechanical allodynia.METHODS:
Male adult SD rats were used for neuropathic pain model. Plasma superoxide production rates of before ischemia (BI) and 5 min after reperfusion (JR) were measured via cytochrome C reduction in the presence of xanthine (without xanthine oxidase, kinetics, 550 nm). Mechanical allodynia was measured in both hindpaws. Activation of NMDA receptor subunit 1 (P-NR1) of lumbar spinal cord (L4-L6) in accordance with the change of allodynia was analyzed by the Western blot.RESULTS:
Allopurinol-inhibitable, xanthine oxidase-mediated plasma superoxide production was increased at AR. Mechanical allodynia was present in both hindpaws as early as 1 hr after reperfusion, and lasted at least 1 week. The expression of P-NR1 was the highest at 3 days after reperfusion when the withdrawal threshold was the lowest point. SOD significantly blocked P-NR1 activation.CONCLUSIONS:
This study suggests that ischemia/reperfusion injury induced neuropathic pain model is a good candidate for the research fields of ROS and pain mechanism. The generation of ROS, especially superoxide is partly responsible for NMDA-mediated mechanical allodynia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasma
/
Spinal Cord
/
Xanthine Oxidase
/
Kinetics
/
Reperfusion
/
Blotting, Western
/
N-Methylaspartate
/
Reactive Oxygen Species
/
Superoxides
/
Xanthine
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Korean Journal of Anesthesiology
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS