Role of Casein Kinase 2 in Parkinsonian Toxin 1-Methyl-4-phenylpyridinium-induced Cell Death
Journal of the Korean Neurological Association
;
: 157-165, 2010.
Article
in Korean
| WPRIM
| ID: wpr-147227
ABSTRACT
BACKGROUND:
Protein casein kinase 2 is involved in signal transduction, cell growth, and apoptosis. However, it has not been elucidated whether parkinsonian toxin 1-methyl-4-phenylpyridinium (MPP+)-induced neuronal cell death is mediated by a casein-kinase-2-mediated pathway.METHODS:
We monitored apoptosis-related protein activation, changes in the level of casein kinase 2, nuclear damage, and apoptosis in differentiated PC12 cells exposed to MPP+ in combination with casein kinase 2 inhibitor.RESULTS:
Casein kinase 2 inhibitors [4,5,6,7-tetrabromobenzotriazole (TBB), 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole, and apigenin] reduced MPP+- and rotenone-induced cell death in differentiated PC12 cells. TBB inhibited the MPP+-induced activation of apoptosis-related proteins (decreases in Bid and Bcl-2 levels, increase in Bax levels, cytochrome c release, and caspase-3 activation), increase in casein kinase 2 levels, and nuclear damage.CONCLUSIONS:
Administering casein kinase 2 inhibitor TBB at concentrations that do not induce toxic effects may reduce MPP+-induced cell death in differentiated PC12 cells by suppressing the apoptosis-related protein activation that leads to cytochrome c release and subsequent activation of caspase-3. The results suggest that MPP+-induced cell death process is mediated by a casein kinase 2 pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Triazoles
/
Signal Transduction
/
Proteins
/
Caseins
/
1-Methyl-4-phenylpyridinium
/
PC12 Cells
/
Cell Death
/
Apoptosis
/
Cytochromes c
/
Casein Kinases
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Neurological Association
Year:
2010
Type:
Article
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