Murine Model of Buckwheat Allergy by Intragastric Sensitization with Fresh Buckwheat Flour Extract
Journal of Korean Medical Science
; : 566-572, 2005.
Article
in En
| WPRIM
| ID: wpr-147625
Responsible library:
WPRO
ABSTRACT
Food allergies affect about 4% of the Korean population, and buckwheat allergy is one of the most severe food allergies in Korea. The purpose of the present study was to develop a murine model of IgE-mediated buckwheat hypersensitivity induced by intragastric sensitization. Young female C3H/HeJ mice were sensitized and challenged intragastricly with fresh buckwheat flour (1, 5, 25 mg/dose of proteins) mixed in cholera toxin, followed by intragastric challenge. Anaphylactic reactions, antigen-specific antibodies, splenocytes proliferation assays and cytokine productions were evaluated. Oral buckwheat challenges of sensitized mice provoked anaphylactic reactions such as severe scratch, perioral/periorbital swellings, or decreased activity. Reactions were associated with elevated levels of buckwheatspecific IgE antibodies. Splenocytes from buckwheat allergic mice exhibited significantly greater proliferative responses to buckwheat than non-allergic mice. Buckwheat-stimulated IL-4, IL-5, and INF-gamma productions were associated with elevated levels of buckwheat-specific IgE in sensitized mice. In this model, 1 mg and 5 mg dose of sensitization produced almost the same degree of Th2-directed immune response, however, a 25 mg dose showed blunted antibody responses. In conclusion, we developed IgE-mediated buckwheat allergy by intragastric sensitization and challenge, and this model could provide a good tool for future studies.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Spleen
/
Stomach
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Time Factors
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Immunoglobulin E
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Immunoglobulin G
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Enzyme-Linked Immunosorbent Assay
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Comparative Study
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Plant Extracts
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T-Lymphocytes
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Interleukin-4
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Journal of Korean Medical Science
Year:
2005
Type:
Article