IL-1beta Acts in Synergy with Endogenous IL-1beta in A375-S2 Human Melanoma Cell Apoptosis Through Mitochondrial Pathway
Journal of Korean Medical Science
;
: 555-561, 2005.
Article
in English
| WPRIM
| ID: wpr-147627
ABSTRACT
Interleukin-1beta (IL-1beta) is a pivotal proinflammatory cytokine. To investigate the mechanism of IL-1beta-induced cell death in human malignant melanoma A375-S2 cells, MTT assay, photomicroscopical observation, DNA agarose gel electrophoresis, radioimmunoassay and Western blot analysis were carried out. IL-1beta did not only induce nuclear condensation and DNA fragmentation, but also increased degradation of two substrates of caspase-3, poly ADP-ribose polymerase (PARP) and inhibitor of caspase-activated DNase (ICAD). Simultaneously, release of precursor of IL-1beta (pro-IL-1beta) and endogenous IL-1beta production were involved in the apoptotic process. IL-1beta enhanced the ratio of Bax/Bcl-2 and Bax/Bcl-xL expression and up-regulated apoptosis inducing factor (AIF) expression, which required the activation of downstream caspases. These results suggest that IL-1beta induces endogenous IL-1beta production, enhances cleavage of caspase downstream substrates and promotes mitochondria mediated apoptosis in A375-S2 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Comparative Study
/
Cell Survival
/
Blotting, Western
/
Interleukin-6
/
Interleukin-1
/
Lymphotoxin-alpha
/
Poly(ADP-ribose) Polymerases
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
Limits:
Humans
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2005
Type:
Article
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