Angiopoietin-1 and Angiopoietin-2 Expression Imbalance Influence in Early Period After Subarachnoid Hemorrhage / 대한배뇨장애요실금학회지
International Neurourology Journal
;
: 288-295, 2016.
Article
in English
| WPRIM
| ID: wpr-148118
ABSTRACT
PURPOSE:
Microvascular endothelial integrity is important for maintaining the blood-brain barrier (BBB). However, subarachnoid hemorrhage (SAH) disrupts this integrity, making the BBB dysfunctional—an important pathophysiological change after SAH. Angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) regulate microvascular permeability by balancing each other’s expression.METHODS:
This study investigated the dynamics of Ang-1 and Ang-2 expression after SAH and the protective effect of Ang-1 on BBB functioning using an endovascular puncture model of rat SAH. The Ang-1 and Ang-2 expression in brain tissue was determined by immunohistochemistry. In addition, Western blotting was used to estimate Ang-1 and Ang-2 concentration and to compare them at 6–72 hours post-SAH cortex and hippocampus. Evans blue viability assay was used to evaluate BBB permeability, and neurological testing was implemented to evaluate neurological impairment during SAH.RESULTS:
It was found that following SAH, Ang-1 expression decreases and Ang-2 expression increases in the cortex, hippocampus, and microvessels. The Ang-1/Ang-2 ratio decreased as quickly as 6 hours after SAH and reached its lowest 1 day after SAH. Finally, it was found that exogenous Ang-1 reduces SAH-associated BBB leakage and improves neurological function in post-SAH rats.CONCLUSIONS:
Our findings suggest that the equilibrium between Ang-1 and Ang-2 is broken in a period shortly after SAH, and the treatment of exogenous Ang-1 injection alleviates neurological dysfunctions through decreasing BBB destruction.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Permeability
/
Subarachnoid Hemorrhage
/
Brain
/
Brain Injuries
/
Capillary Permeability
/
Immunohistochemistry
/
Blood-Brain Barrier
/
Punctures
/
Blotting, Western
/
Angiopoietin-1
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
International Neurourology Journal
Year:
2016
Type:
Article
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