Induction of castration by immunization of male dogs with recombinant gonadotropin-releasing hormone (GnRH)-canine distemper virus (CDV) T helper cell epitope p35
Journal of Veterinary Science
;
: 21-24, 2005.
Article
in English
| WPRIM
| ID: wpr-148131
ABSTRACT
Immunocastration is a considerable alternative to a surgical castration method especially in male animal species for alleviating unwanted male behaviors and characteristics. Induction of high titer of antibody specific for gonadotropin-releasing hormone (GnRH) correlates with the regression of testes. Fusion proteins composed of canine GnRH and T helper (Th) cell epitope p35 originated from canine distemper virus (CDV) F protein and goat rotavirus VP6 protein were produced in E. coli. When these fusion proteins were injected to male dogs which were previously immunized with CDV vaccine, the fusion protein of GnRH-CDV Th cell epitope p35 induced much higher antibody than that of GnRH-rotavirus VP6 protein or GnRH alone. The degeneration of spermatogenesis was also verified in the male dogs immunized with the fusion protein of GnRH-CDV Th cell epitope p35. These results indicate that canine GnRH conjugated to CDV Th cell epitope p35 acted as a strong immunogen and the antibody to GnRH specifically neutralized GnRH in the testes. This study also implies a potential application of GnRH-based vaccines for immunocastration of male pets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Size
/
Spermatogenesis
/
Testis
/
Recombinant Proteins
/
Molecular Sequence Data
/
Base Sequence
/
Gonadotropin-Releasing Hormone
/
Amino Acid Sequence
/
T-Lymphocytes, Helper-Inducer
/
Epitopes, T-Lymphocyte
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2005
Type:
Article
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