Dose Optimization for Single Intradiscal Administration of the Tumor Necrosis Factor-α Inhibitor, Etanercept, in Rat Disc Injury Models
Asian Spine Journal
;
: 619-623, 2016.
Article
in English
| WPRIM
| ID: wpr-148238
ABSTRACT
STUDY DESIGN:
Experimental animal study.PURPOSE:
We aimed to determine the optimal dose of a single direct injection of the tumor necrosis factor (TNF)-α inhibitor, etanercept, by using the rat model of degenerative intervertebral disc from injury. OVERVIEW OF LITERATURE The pain-related peptide expression was suppressed in the etanercept (100 µg and 1,000 µg)-administered groups in a dose-dependent manner.METHODS:
The neurotracer FluoroGold (FG) was applied to the surfaces of L4/5 discs to label their innervating dorsal root ganglion (DRG) neurons (n=50). Ten rats were included in the nonpunctured disc sham surgery control group, whereas the other 40 were included in the experimental group in which intervertebral discs were punctured with a 23-gauge needle. Saline or etanercept (10 µg, 100 µg, or 1,000 µg) was injected into the punctured discs (n=10 for each treatment). After 14 days of surgery, DRGs from L1 to L6 were harvested, sectioned, and immunostained for calcitonin gene-related peptide (CGRP). The proportion of FG-labeled CGRP-immunoreactive DRG neurons was evaluated in all the groups.RESULTS:
There were no significant differences between the puncture+saline group and the puncture+10-µg etanercept group (p >0.05). However, a significant decrease in the percentage of FG and CGRP double-positive cells in FG-positive cells was observed in the etanercept (100 µg and 1,000 µg)-administered groups in a dose-dependent manner (p <0.05).CONCLUSIONS:
When a low dose of the TNF-α inhibitor (10 µg of etanercept) was directly administered to the rat intervertebral disc in the rat model of degenerative intervertebral disc from injury, no suppressive effect on the pain-related peptide expression was observed. However, when a higher dose of etanercept (100 µg and 1,000 µg) was administered, the pain-related peptide expression was suppressed in a dose-dependent manner.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Calcitonin Gene-Related Peptide
/
Tumor Necrosis Factor-alpha
/
Diagnosis-Related Groups
/
Models, Animal
/
Intervertebral Disc Degeneration
/
Etanercept
/
Ganglia, Spinal
/
Intervertebral Disc
/
Necrosis
/
Needles
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Asian Spine Journal
Year:
2016
Type:
Article
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