Effects of echinomycin on endothelin-2 expression and ovulation in immature rats primed with gonadotropins
Experimental & Molecular Medicine
;
: 615-621, 2012.
Article
in English
| WPRIM
| ID: wpr-14961
ABSTRACT
Echinomycin is a small-molecule inhibitor of hypoxia-inducible factor-1 DNA-binding activity, which plays a crucial role in ovarian ovulation in mammalians. The present study was designed to test the hypothesis that hypoxia-inducible factor (HIF)-1alpha-mediated endothelin (ET)-2 expressions contributed to ovarian ovulation in response to human chorionic gonadotropin (hCG) during gonadotropin-induced superuvulation. By real-time RT-PCR analysis, ET-2 mRNA level was found to significantly decrease in the ovaries after echinomycin treatment, while HIF-1alpha mRNA and protein expression was not obviously changed. Further analysis also showed that these changes of ET-2 mRNA were consistent with HIF-1 activity in the ovaires, which is similar with HIF-1alpha and ET-2 expression in the granulosa cells with gonadotropin and echinomycin treatments. The results of HIF-1alpha and ET-2 expression in the granulosa cells transfected with cis-element oligodeoxynucleotide (dsODN) under gonadotropin treatment further indicated HIF-1alpha directly mediated the transcriptional activation of ET-2 during gonadotropin-induced superuvulation. Taken together, these results demonstrated that HIF-1alpha-mediated ET-2 transcriptional activation is one of the important mechanisms regulating gonadotropin-induced mammalian ovulatory precess in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligonucleotides
/
Ovary
/
Superovulation
/
Transcriptional Activation
/
Cells, Cultured
/
Gonadotropins, Equine
/
Rats, Sprague-Dawley
/
Endothelin-2
/
Echinomycin
/
Hypoxia-Inducible Factor 1, alpha Subunit
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2012
Type:
Article
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