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Degranulation of human eosinophils induced by Paragonimus westermani-secreted protease
Article in English | WPRIM | ID: wpr-14971
Responsible library: WPRO
ABSTRACT
Eosinophil degranulation is considered to be a key effector function for the killing of helminthic worms and tissue inflammation at worm-infected lesion sites. However, relatively little data are available with regard to eosinophil response after stimulation with worm-secreted products which contain a large quantity of cysteine proteases. In this study, we attempted to determine whether the degranulation of human eosinophils could be induced by the direct stimulation of the excretory-secretory products (ESP) of Paragonimus westermani, which causes pulmonary paragonimiasis in human beings. Incubation of eosinophils for 3 hr with Paragonimus-secreted products resulted in marked degranulation, as evidenced by the release of eosinophil-derived neurotoxin (EDN) in the culture supernatants. Moreover, superoxide anion was produced by eosinophils after stimulation of the ESP. The ESP-induced EDN release was found to be significantly inhibited when the ESP was pretreated with protease inhibitor cocktail or the cysteine protease inhibitor, E-64. These findings suggest that human eosinophils become degranulated in response to P. westermani-secreted proteases, which may contribute to in vivo tissue inflammation around the worms.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Time Factors / Humans / Cysteine Endopeptidases / Cell Degranulation / Superoxides / Paragonimus westermani / Eosinophil-Derived Neurotoxin / Eosinophils / Animals Language: English Journal: The Korean Journal of Parasitology Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Time Factors / Humans / Cysteine Endopeptidases / Cell Degranulation / Superoxides / Paragonimus westermani / Eosinophil-Derived Neurotoxin / Eosinophils / Animals Language: English Journal: The Korean Journal of Parasitology Year: 2005 Type: Article