Degranulation of human eosinophils induced by Paragonimus westermani-secreted protease
The Korean Journal of Parasitology
;
: 33-37, 2005.
Article
in English
| WPRIM
| ID: wpr-14971
ABSTRACT
Eosinophil degranulation is considered to be a key effector function for the killing of helminthic worms and tissue inflammation at worm-infected lesion sites. However, relatively little data are available with regard to eosinophil response after stimulation with worm-secreted products which contain a large quantity of cysteine proteases. In this study, we attempted to determine whether the degranulation of human eosinophils could be induced by the direct stimulation of the excretory-secretory products (ESP) of Paragonimus westermani, which causes pulmonary paragonimiasis in human beings. Incubation of eosinophils for 3 hr with Paragonimus-secreted products resulted in marked degranulation, as evidenced by the release of eosinophil-derived neurotoxin (EDN) in the culture supernatants. Moreover, superoxide anion was produced by eosinophils after stimulation of the ESP. The ESP-induced EDN release was found to be significantly inhibited when the ESP was pretreated with protease inhibitor cocktail or the cysteine protease inhibitor, E-64. These findings suggest that human eosinophils become degranulated in response to P. westermani-secreted proteases, which may contribute to in vivo tissue inflammation around the worms.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Cysteine Endopeptidases
/
Cell Degranulation
/
Superoxides
/
Paragonimus westermani
/
Eosinophil-Derived Neurotoxin
/
Eosinophils
Limits:
Animals
/
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2005
Type:
Article
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