Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii
The Korean Journal of Parasitology
;
: 27-32, 2005.
Article
in English
| WPRIM
| ID: wpr-14972
ABSTRACT
Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5' and 3' ends of SAG2, respectively, with Mse I, Sau3A I, and Hha I, revealed that KI-1 is included in the genotype I, which includes the worldwide virulent RH strain. In addition, when the whole sequences of the coding regions of SAG1, rhoptry antigen 1 (ROP1), and GRA8 genes of KI-1 were compared with those of RH, minor nucleotide polymorphisms and amino acid substitutions were identified. These results show that KI-1 is a new geographical strain of T. gondii that can be included in the genotype I.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
Toxoplasma
/
Base Sequence
/
RNA, Protozoan
/
Amino Acid Sequence
/
Genes, Protozoan
/
Genotype
/
Antigens, Protozoan
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2005
Type:
Article
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