NaF-induced Autophagy on SCC25 Human Tongue Squamous Cell Carcinoma Cells
International Journal of Oral Biology
;
: 193-199, 2014.
Article
in English
| WPRIM
| ID: wpr-149980
ABSTRACT
Fluoride has been accepted as an important material for oral health and is widely used to prevent dental caries in dentistry. However, its safety is still questioned by some. Autophagy has been implicated in cancer cell survival and death, and may play an important role in oral cancer. This study was undertaken to examine whether sodium fluoride (NaF) modulates autophagy in SCC25 human tongue squamous cell carcinoma cells. NaF demonstrated anticancer activity via autophagic and apoptotic cell death. Autophagic vacuoles were detectable using observed to form by monodansylcadaverine (MDC) and acridine orange (AO). Analysis of NaF-treated SCC25 cells for the presence of biochemical markers revealed direct effects on the conversion of LC-3II, degradation of p62/SQSTM1, cleavage formation of ATG5 and Beclin-1, and caspase activation. NaF-induced cell death was suppressed by the autophagy inhibitor 3-methyladenine (3-MA). NaF-induced autophagy was confirmed as a pro-death signal in SCC25 cells. These results implicate NaF as a novel anticancer compound for oral cancer therapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sodium Fluoride
/
Autophagy
/
Tongue
/
Vacuoles
/
Acridine Orange
/
Mouth Neoplasms
/
Carcinoma, Squamous Cell
/
Biomarkers
/
Cell Survival
/
Oral Health
Limits:
Humans
Language:
English
Journal:
International Journal of Oral Biology
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS