A Gene and Neural Stem Cell Therapy Platform Based on Neuronal Cell Type-Inducible Gene Overexpression
Yonsei Medical Journal
;
: 1036-1043, 2015.
Article
in English
| WPRIM
| ID: wpr-150480
ABSTRACT
PURPOSE:
Spinal cord injury (SCI) is associated with permanent neurological damage, and treatment thereof with a single modality often does not provide sufficient therapeutic outcomes. Therefore, a strategy that combines two or more techniques might show better therapeutic effects. MATERIALS ANDMETHODS:
In this study, we designed a combined treatment strategy based on neural stem cells (NSCs) introduced via a neuronal cell type-inducible transgene expression system (NSE) controlled by a neuron-specific enolase (NSE) promoter to maximize therapeutic efficiency and neuronal differentiation. The luciferase gene was chosen to confirm whether this combined system was working properly prior to using a therapeutic gene. The luciferase expression levels of NSCs introduced via the neuronal cell type-inducible luciferase expression system (NSELuci) or via a general luciferase expressing system (SVLuci) were measured and compared in vitro and in vivo.RESULTS:
NSCs introduced via the neuronal cell type-inducible luciferase expressing system (NSELuci-NSCs) showed a high level of luciferase expression, compared to NSCs introduced via a general luciferase expressing system (SVLuci-NSCs). Interestingly, the luciferase expression level of NSELuci-NSCs increased greatly after differentiation into neurons.CONCLUSION:
We demonstrated that a neuronal cell type-inducible gene expression system is suitable for introducing NSCs in combined treatment strategies. We suggest that the proposed strategy may be a promising tool for the treatment of neurodegenerative disorders, including SCI.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphopyruvate Hydratase
/
Spinal Cord Injuries
/
Stem Cells
/
Genetic Therapy
/
Gene Expression
/
Cell Differentiation
/
Promoter Regions, Genetic
/
Gene Regulatory Networks
/
Neural Stem Cells
/
Luciferases
Limits:
Humans
Language:
English
Journal:
Yonsei Medical Journal
Year:
2015
Type:
Article
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