Effects of Pupil Dilation and Constriction Agents on Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
;
: 1089-1093, 2011.
Article
in Korean
| WPRIM
| ID: wpr-15074
ABSTRACT
PURPOSE:
To investigate the effects of pupil dilation and constriction agents on the survival and production of nitric oxide (NO) in cultured human trabecular meshwork cells (HTMC).METHODS:
Primarily cultured HTMC were exposed to 0, 0.01, and 0.1 mg/ml of tropicamide, cyclopentolate, atropine, or pilocarpine for 2 hours. Cellular survival and production of NO were assessed using the MTT assay and Griess assay, respectively.RESULTS:
Tropicamide, cyclopentolate, atropine, and pilocarpine decreased cellular survival at the concentration of 0.1 mg. At the concentration of 0.01 mg/ml, all agents decreased production of NO to some extent, although the reduction was not statistically significant.CONCLUSIONS:
Pupil dilation and constriction agents may be toxic to HTMC if used at high concentrations or if used frequently in the short-term but may not affect trabecular outflow.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pilocarpine
/
Atropine
/
Trabecular Meshwork
/
Tropicamide
/
Pupil
/
Constriction
/
Cyclopentolate
/
Nitric Oxide
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2011
Type:
Article
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