Identification and purification of IgE-reactive proteins in German cockroach extract
Yonsei Medical Journal
;
: 283-289, 1999.
Article
in English
| WPRIM
| ID: wpr-150895
ABSTRACT
Cockroaches have been implicated as a cause of respiratory allergy in urban areas worldwide. IgE-reactive German cockroach proteins were identified with molecular weights (MWs) of 90, 66, 50, 43 and 36 KD by immunoblot analysis in both immune BALB/c mice and sensitized humans. Prominent IgE-reactive proteins were purified using FPLC by ion-exchange chromatography, gel filtration and hydrophobic chromatography. The N-terminal amino acid sequence of a purified protein with a MW of 66 KD on SDS-PAGE was Val-Thr-Leu-Lys-Lys(Val)-Met-Ile-Lys-Thr-Phe-Tyr. No homologous protein was found through a search of GenBank that indicated a novel IgE-reactive protein in German cockroach extract. Another purified protein with a MW of 36 KD reacted strongly with a monoclonal antibody against Bla g 2.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tissue Extracts
/
Immunoglobulin E
/
Amino Acid Sequence
/
Cockroaches
/
Insect Proteins
/
Animals
/
Mice, Inbred BALB C
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Yonsei Medical Journal
Year:
1999
Type:
Article
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