Effect of Fixation Time and Freeze-thaw Cycles on the Molecular Analysis of Viral DNA
Journal of Veterinary Science
;
: 203-204, 2003.
Article
in English
| WPRIM
| ID: wpr-151143
ABSTRACT
This study was done to determine the effect of fixation time and freeze-thaw (FT) cycles on the quantitation and positivity of viral DNA. There were significant decreases in the viral DNA copies in the specimens subjected to formalin fixation more than 24 hours. However, The viral DNAs of specimens with FT cycles were not remarkably decreased as compare with those of fresh samples. These results implicate that there may be need to fix the samples less than 24 hours. Also, results of retrospective studies performed on specimens subjected to long-term fixation may be compromised.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Virology
/
DNA, Viral
/
Polymerase Chain Reaction
/
Freezing
/
Herpesviridae
Type of study:
Observational study
Language:
English
Journal:
Journal of Veterinary Science
Year:
2003
Type:
Article
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