Change of alpha-SM Actin Expression Induced by the Antibody for TGF-beta in Fibroblast NIH3T3 Culture: The basic research for the inhibition of wound contracture
Journal of the Korean Society of Coloproctology
;
: 113-118, 2001.
Article
in Korean
| WPRIM
| ID: wpr-151299
ABSTRACT
PURPOSE:
The purpose of this experiment is to measure the expression of TGF-beta and alpha-SM actin (smooth muscle actin) from fibroblast culture by the duration of culture days and to analyze the inhibition of alpha-SM actin expression in fibroblast by the antibodies for TGF-beta.METHODS:
The levels of alpha-SM actin from the paired NIH3T3 cell cultures with TGF-beta 1 containing medium (10 ng/ml) and with the antibody (for TGF-beta) medium (1 or 2 ug/ml) were determined by SDS PAGE for cell lysate protein, Western blot with ECL autoradiography and immuno - slot blot.RESULTS:
In NIH3T3 culture, the expression of alpha-SM actin increased at culture days 4, 5, 6. TGF-beta was expressed from 2nd day of culture and increased by day 7. The addition of TGF beta (10 ng/ml) did not increased the expression of alpha-SM actin. But alpha-SM actin expression decreased in the presence of anti-TGF beta antibody. The decrease of expression was proportional to the concentration of antibody and duration of exposure to the antibody.CONCLUSIONS:
Endogenous TGF-beta produced by fibroblast cultures is sufficient to express the alpha-SM actin from the myofiboblast. There was no additive expression of alpha-SM actin with exogenous TGF-beta 1. The antibody for TGF- beta inhibits the production of the alpha-SM actin during wound healing and may prevent the wound contracture.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Autoradiography
/
Wound Healing
/
Wounds and Injuries
/
Blotting, Western
/
Transforming Growth Factor beta
/
Actins
/
Cell Culture Techniques
/
Contracture
/
Electrophoresis, Polyacrylamide Gel
/
Myofibroblasts
Language:
Korean
Journal:
Journal of the Korean Society of Coloproctology
Year:
2001
Type:
Article
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