Role of protease inhibitors and acylation stimulating protein in the adipogenesis in 3T3-L1 cells
Journal of Veterinary Science
;
: 197-201, 2009.
Article
in English
| WPRIM
| ID: wpr-151426
ABSTRACT
Treatment of AIDS (HIV) and hepatitis C virus needs protease inhibitors (PI) to prevent viral replication. Uses of PI in therapy are usually associated with a decrease in body weight and dyslipidemia. Acylation stimulating protein (ASP) is a protein synthesized in adipocytes to increase triglycerides biosynthesis, for that the relation of PI and ASP to adipogenesis is tested in this work. ASP expression was increased during 3T3-L1 differentiation and reached a peak at day 8 with cell maturation. Addition of PI during adipocytes differentiation dose dependently and significantly (p < 0.5) inhibited the degree of triglycerides (TG) accumulation. Moreover, presence of ASP (450 ng/mL) in media significantly (p < 0.5) stimulated the degree of TG accumulation and there was additive stimulation for ASP when added with insulin (10 microgram/mL). Finally, when ASP in different doses (Low, 16.7; Medium, 45 and High, 450 ng/mL) incubated with a dose of x150 PI, ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation. The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protease Inhibitors
/
Time Factors
/
Gene Expression Regulation
/
3T3 Cells
/
Intercellular Signaling Peptides and Proteins
/
Adipogenesis
/
Lipid Metabolism
/
Hypoglycemic Agents
/
Insulin
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2009
Type:
Article
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