Evaluation of Cysticidal Effects of Contact Lens Disinfectant on Acanthamoeba Isolates
Journal of the Korean Ophthalmological Society
;
: 431-437, 2007.
Article
in Korean
| WPRIM
| ID: wpr-151480
ABSTRACT
PURPOSE:
To evaluate the cysticidal effect of 5 kinds of commercially available contact lens disinfectants against 2 clinical isolates of Acanthamoeba.METHODS:
Five kinds of commercially available contact lens disinfectants were soaked with cysts of Acanthamoeba ludgdunesis and castellanii at the concentration of 10(3), 10(4), and 10(5) cells/ml for 1 and 4 or 6 hours. Cysts which were not excysted in 7 days after treatment were recognized to be killed. Morphologic changes were evaluated by electron microscopic observation.RESULTS:
Contact lens disinfectants which contain myristamidopropyl dimethylamine (MAPD) showed the best cysticidal effect. These disinfectants demonstrated a cysticidal effect on both Acanthamoeba species of all concentrations in 6-hour treatment. Contact lens disinfectants which contain polyhexamethylene biguanide (PHMB) did not demonstrate cysticidal effect, except for Acanthamoeba castellanii at the concentration of 10(3) cells/ml, in either 4- or 6-hour treatment. Separation of plasma membrane from endocyst and damage of organelles were prominent in cases showing a cysticidal effect.CONCLUSIONS:
Contact lens disinfectant which contains MAPD may be helpful in preventing the Acanthamoeba keratitis. A higher concentration of PHMB is required to be effective in preventing Acanthamoeba keratitis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Acanthamoeba
/
Organelles
/
Acanthamoeba Keratitis
/
Cell Membrane
/
Contact Lens Solutions
/
Acanthamoeba castellanii
/
Disinfectants
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2007
Type:
Article
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