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Xpert CARBA-R Assay for the Detection of Carbapenemase-Producing Organisms in Intensive Care Unit Patients of a Korean Tertiary Care Hospital
Annals of Laboratory Medicine ; : 162-165, 2016.
Article in English | WPRIM | ID: wpr-151581
ABSTRACT
Carbapenemase-producing organisms (CPO) are rapidly disseminating worldwide, and their presence in tertiary care hospitals poses a significant threat to the management of nosocomial infections. There is a need to control CPO, especially in intensive care unit (ICU) patients, because these organisms are resistant to most beta-lactam antibiotics and are easily transmitted. At present, the identification of CPO is time-consuming; hence, this study focused on the use of the Xpert CARBA-R assay (Cepheid, USA) to determine intestinal colonization rates of CPO in patients admitted to the ICU of a tertiary care hospital in Korea. Forty clinical stool samples were collected and inoculated both in a CARBA-R cartridge and in conventional culture plates. The CARBA-R assay required only ~one hour to screen CPO, while the time required for conventional culture was over three days. We also found that the prevalences of intestinal colonization by carbapenem-resistant organisms and Enterobacteriaceae were 17.5% (7 out of 40) and 7.5% (3 out of 40), respectively. Among the colonizing strains, three that contained carbapenemase, including Klebsiella pneumonia carbapenemase (KPC), and imipenem (IMP) and Verona integron-mediated metallo-beta-lactamase (VIM) were found. With its convenience, the Xpert CARBA-R assay can be included in CPO surveillance strategies.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Reagent Kits, Diagnostic / Bacterial Proteins / Beta-Lactamases / Tertiary Healthcare / DNA, Bacterial / Imipenem / Drug Resistance, Multiple, Bacterial / Enterobacteriaceae / Feces / Republic of Korea Type of study: Diagnostic study Limits: Humans Country/Region as subject: Asia Language: English Journal: Annals of Laboratory Medicine Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Reagent Kits, Diagnostic / Bacterial Proteins / Beta-Lactamases / Tertiary Healthcare / DNA, Bacterial / Imipenem / Drug Resistance, Multiple, Bacterial / Enterobacteriaceae / Feces / Republic of Korea Type of study: Diagnostic study Limits: Humans Country/Region as subject: Asia Language: English Journal: Annals of Laboratory Medicine Year: 2016 Type: Article