Comparison of Rapid Antigen Test and Real-Time Reverse Transcriptase PCR for Diagnosing Novel Swine Influenza A (H1N1) / 대한임상미생물학회지
Korean Journal of Clinical Microbiology
;
: 109-113, 2010.
Article
in Korean
| WPRIM
| ID: wpr-152159
ABSTRACT
BACKGROUND:
Novel swine influenza (H1N1) was first identified in Mexico in April 2009. Because of its high infectivity and worldwide distribution, a rapid and efficient screening test is necessary. Here we evaluated the usefulness of a rapid antigen test currently in use, compared to real-time RT-PCR (rRT-PCR) as a screening test for detection of novel swine influenza (H1N1).METHODS:
A total of 1,228 patients who visited Hallym University Kangdong Sacred Heart Hospital with influenza-like illness between 14 August 2009 and 30 September 2009, and were tested by both rapid antigen and rRT-PCR tests, were enrolled in this study.RESULTS:
Sensitivity, specificity, predictive value of a positive test, and predictive value of a negative test for the rapid antigen test were 30.5%, 99.2%, 86.4% and 90.1%, respectively. Fifty-one (4.2%) patients were positive for both rapid antigen test and rRT-PCR, and 1,053 (85.7%) were negative for both rapid antigen test and rRT-PCR. A total of 124 (10.1%) patients showed a discrepancy between the two tests. Among them, 116 (9.4%) were only positive for rRT-PCR and 8 (0.7%) were only positive for the rapid antigen test. The latter 8 patients all showed negative H1/M2 results in rRT-PCR. There were significant differences in detection rates of the rapid antigen test between different H1 Ct (threshold cycle) interval groups and for different age groups (P<0.05).CONCLUSION:
Although the rapid antigen test is easy to perform and provides fast results, its limits as a screening test for detection of novel swine influenza (H1N1) due to its low sensitivity compared to rRT-PCR need to be considered in practical situations.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Mass Screening
/
Sensitivity and Specificity
/
RNA-Directed DNA Polymerase
/
Reverse Transcriptase Polymerase Chain Reaction
/
Influenza, Human
/
Real-Time Polymerase Chain Reaction
/
Heart
/
Mexico
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Country/Region as subject:
Mexico
Language:
Korean
Journal:
Korean Journal of Clinical Microbiology
Year:
2010
Type:
Article
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