Pro-inflammatory cytokine expression in human gingival fibroblasts by Tannerella forsythia whole bacteria, membrane proteins, and lipopolysaccharide / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 543-550, 2008.
Article
in Korean
| WPRIM
| ID: wpr-152425
ABSTRACT
PURPOSE:
The purpose of this study was to investigate induction of cytokine expression in human gingival fibroblasts (HGFs) by whole cell and the components of T. forsythia. MATERIAL ANDMETHODS:
After HGFs were treated with lipopolysaccharide (LPS), membrane protein isolated from T. forsythia or culture media of T. forsythia, the induction of interleukin (IL)-1, IL-6 and IL-8 was examined with real-time PCR and ELISA. Their induction ability of cytokines was compared with whole bacteria.RESULT:
The expression of IL-6 and IL-8 was significantly induced in HGFs by whole bacteria and membrane protein. The expression of IL-1beta was induced by membrane protein of T. forsythia, not by whole bacteria. LPS and condition media of T. forsythia slightly activated HGFs.CONCLUSION:
The membrane protein of T. forsythia could be one of virulence factors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacteria
/
Enzyme-Linked Immunosorbent Assay
/
Cytokines
/
Interleukin-8
/
Interleukins
/
Interleukin-6
/
Culture Media
/
Forsythia
/
Virulence Factors
/
Real-Time Polymerase Chain Reaction
Limits:
Humans
Language:
Korean
Journal:
The Journal of the Korean Academy of Periodontology
Year:
2008
Type:
Article
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