The Apoptosis and Expression of p53, Bcl-2 in Graded Contusion Injury of Rat Spinal Cord
Journal of Korean Neurosurgical Society
;
: 317-323, 2004.
Article
in English
| WPRIM
| ID: wpr-153088
ABSTRACT
OBJECTIVE:
This study is designed to investigate how apoptosis is presented and how the genes of p53 and bcl-2 are expressed depending on graded injury in experimental spinal cord injury.METHODS:
Experimental spinal cord injury was made on rats with weight drop method. Two different amounts of impact were applied on rat spinal cord. Rats were categorized into three groups (control; five rats, mild injury; five rats, severe injury; five rats). Fourty eight hours following cord injury, cord specimen was harvested from injury epicenter. TUNEL staining was done for apoptotic detection and immunohistochemical staining for p53 and bcl-2 expression. Positively stained cells were counted and mean values were compared among three groups.RESULTS:
TUNEL positive cells increased depending on injury severity(p=0.027). The p53 positive cells increased in both injury groups compared to control group(p=0.001). Bcl-2 positive cells decreased as injury amount increased(p=0.002). The p53 expression increased in proportion to TUNEL staining in correlation curve in white matter(correlation coefficient, 0.387). The bcl-2 expression was inversely proportional to TUNEL staining and steeper decrease was found in gray matter than in white matter (correlation coefficient, -0.875).CONCLUSION:
Apoptosis increases as the injury grading elevated within 20gm-cm of impact. The p53 seems to promote apoptosis in white matter, but do not show proportional relationship with injury amount. Bcl-2 appeared to be protective to cell death due to apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spinal Cord
/
Spinal Cord Injuries
/
Cell Death
/
Apoptosis
/
Contusions
/
In Situ Nick-End Labeling
Limits:
Animals
Language:
English
Journal:
Journal of Korean Neurosurgical Society
Year:
2004
Type:
Article
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