Induction of Angiogenic Cytokines in Cultured RPE by Oxidative Stress
Journal of the Korean Ophthalmological Society
;
: 1742-1749, 2004.
Article
in Korean
| WPRIM
| ID: wpr-153102
ABSTRACT
PURPOSE:
This study was undertaken to investigate whether expression of angiogenic cytokines including IL-8 and MCP-1 is induced in the cultured RPE by oxidative stress.METHODS:
ARPE-19 cells were exposed to H2O2 and paraquat for 24, 48, and 72 hours and then assayed for the detection of IL-8 and monocyte chemotactic protein (MCP)-1 expression by reverse transcriptase polymerase chain reaction (RT-PCR) method. To determine whether the antioxidant overcame the oxidative stress, we observed expression of IL-8 and MCP-1 gene after ARPE cells were treated by paraquat and antioxidants including N-acetylcysteine (NAC) and pyrrolidine dithiocarbamate (PDTC) at the same time for 72hr.RESULTS:
Expressions of the angiogenic cytokines, IL-8 and MCP-1 were increased by incubating with paraquat for 72hr. Antioxidants including NAC and PDTC decreased the expression of IL-8 and MCP-1 gene by counteracting the paraquat effect.CONCLUSIONS:
Oxidative stress stimulates angiogenic cytokines including IL-8 and MCP-1 in the cultured RPE. These results may be significant for understanding the cause of age-related macular degeneration and its treatment.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Paraquat
/
Acetylcysteine
/
Monocytes
/
Cytokines
/
Interleukin-8
/
Oxidative Stress
/
Reverse Transcriptase Polymerase Chain Reaction
/
Macular Degeneration
/
Antioxidants
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2004
Type:
Article
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