Proteomic Analysis of Hepatic Ischemia and Reperfusion Injury in Mice / 한국실험동물학회지
Laboratory Animal Research
;
: 69-74, 2010.
Article
in Korean
| WPRIM
| ID: wpr-153259
ABSTRACT
Hepatic ischemia/reperfusion (I/R) injury is an inevitable consequence during liver surgery. I/R injury induces serious hepatic dysfunction and failure. In this study, we identified proteins that were differentially expressed between sham and I/R injured livers. Animals were subjected to hepatic ischemia for 1 hr and were sacrificed at 3hr after reperfusion. Serum ALT and AST levels were significantly increased in I/R-operated animals compared to those of sham-operated animals. Ischemic hepatic lobes of I/R-operated animals showed the hepatic lesion with unclear condensation and sinusoidal congestion. Proteins from hepatic tissue were separated using two dimensional gel electrophosresis. Protein spots with a greater than 2.5-fold change in intensity were identified by mass spectrometry. Among these proteins, glutaredoxin-3, peroxiredoxin-3, glyoxalase I, spermidine synthase, dynamin-1-like protein, annexin A4, eukaryotic initiation factor 3, eukaryotic initiation factor 4A-I, 26S proteasome, proteasome alpha 1, and proteasome beta 4 levels were significantly decreased in I/R-operated animals compared to those of sham-operated animals. These proteins are related to protein synthesis, cellular growth and stabilization, anti-oxidant action. Moreover, Western blot analysis confirmed that dynamin-1-like protein levels were decreased in I/R-operated animals. Our results suggest that hepatic I/R induces the hepatic cells damage by regulation of several proteins.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spermidine Synthase
/
Mass Spectrometry
/
Reperfusion
/
Reperfusion Injury
/
Proteins
/
Peptide Initiation Factors
/
Salicylamides
/
Blotting, Western
/
Annexin A4
/
Estrogens, Conjugated (USP)
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Laboratory Animal Research
Year:
2010
Type:
Article
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