Localization of Intracellular IVIonoclonal Antibody Specific for IVlycobacteria in Experimentally Induced Pulmonary Tuberculosis
Journal of the Korean Radiological Society
;
: 267-271, 1994.
Article
in Korean
| WPRIM
| ID: wpr-153405
ABSTRACT
PURPOSE:
To determine whether intracellular localization of intravenously injected infection specific MoAb can be localized in the infected cells, immunohistochemical staining was obtained in animals infected with Mycobacterium tuberculosis. MATERIALS ANDMETHODS:
One mg of intact mouse MoAb against mwobacteria (group I, n=10) and F(ab')2 (group II, n=6) were intravenously injected to the rabbits of each groups infected with M. tuberculosis H37RV. Immunohistochemical staining using an anti-mouse antibody was obtained at the 1,3, 5, 7 and 8th days in Group I and at the 1, 2, 3rd days in group II by streptavidin biotin method. For the control study, I mg of non specific polyclonal human IgG (group III, n=10) and 100 microgram of normal rabbit IgG F(ab')2 (group IV, n=6) were injected to the rabbits and guinea pigs respectively.RESULTS:
Both groups (group I & II) showed a positive Ag-Ab reaction within the cytoplasm of monocytes. A weak but positive reaction was observed intracellularly in group III, however no positive reaction in group IV.CONCLUSION:
Our results suggest intracellular Ag-Ab reaction plays an important role in the localization of infection by immunoscintigraphy using a specific MoAb fragments.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tuberculosis
/
Tuberculosis, Pulmonary
/
Biotin
/
Immunoglobulin G
/
Monocytes
/
Streptavidin
/
Cytoplasm
/
Guinea Pigs
/
Mycobacterium tuberculosis
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of the Korean Radiological Society
Year:
1994
Type:
Article
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