Granulocyte Stimulating Factor Attenuates Hypoxic-ischemic Brain Injury by Inhibiting Apoptosis in Neonatal Rats
Yonsei Medical Journal
;
: 836-842, 2008.
Article
in English
| WPRIM
| ID: wpr-153692
ABSTRACT
PURPOSE:
This study was undertaken to determine the neuroprotective effect of granulocyte stimulating factor (G-CSF) on neonatal hypoxic-ischemic brain injury. MATERIALS ANDMETHODS:
Seven-day-old male newborn rat pups were subjected to 110 minutes of 8% oxygen following a unilateral carotid artery ligation. Apoptosis was identified by performing terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining and flow cytometry with a combination of fluorescinated annexin V and propidium iodide (PI) and JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide). The extent of cerebral infarction was evaluated at 2 weeks after recovery.RESULTS:
With a single dose (50microgram/kg) of G-CSF treatment immediately after hypoxic-ischemic insult, hypoxia-ischemia induced increase in TUNEL-positive cells, annexinV+/PI- and JC-1 positive apoptotic cells in the ipsilateral cerebral cortex was significantly reduced at 24 hours, measured by flow cytometry, and the extent of cerebral infarction at 2 weeks after recovery was also significantly attenuated compared to the hypoxia-ischemia control group.CONCLUSION:
Our data suggest that G-CSF is neuroprotective by inhibiting apoptosis, thereby reducing the ensuing cerebral infarction in a newborn rat pup model of cerebral hypoxia-ischemia (HI).
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Size
/
Brain
/
Weight Gain
/
Cerebral Infarction
/
Granulocyte Colony-Stimulating Factor
/
Rats, Sprague-Dawley
/
Apoptosis
/
Protective Agents
/
In Situ Nick-End Labeling
/
Hypoxia-Ischemia, Brain
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Yonsei Medical Journal
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS