Development and Evaluation of Indirect ELISA for Detection of Antibodies to Getah Virus in Horse Serum
Journal of Bacteriology and Virology
;
: 63-70, 2016.
Article
in English
| WPRIM
| ID: wpr-153899
ABSTRACT
Getah virus (GETV) is a member of the genus Alphavirus in the family Togaviridae. GETV infection can occur in a wide range of vertebrate species, and the virus has been known for a pathogen of horses and pigs. To rapidly and accurately diagnose GETV infection of a racehorse, an indirect ELISA (I-ELISA) was developed in the present study for detection of antibodies to GETV in serum samples. To evaluate the developed I-ELISA, a total of 240 serum samples from Thoroughbred racehorses raised in Korea were screened in parallel by a serum neutralization (SN) test. The developed I-ELISA exhibited an efficacy comparable to that of the SN test in terms of a high diagnostic sensitivity (86.3%) and specificity (94.5%) at a cut-off absorbance value of 0.25. In addition, our results showed that the developed I-ELISA had a significant correlation with the SN test (r = 0.91; p < 0.05). Taken together, our findings suggest that the I-ELISA developed in this study is a valuable diagnostic tool for the screening of horses suspected to be infected with GETV.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Togaviridae
/
Vertebrates
/
Enzyme-Linked Immunosorbent Assay
/
Mass Screening
/
Sensitivity and Specificity
/
Alphavirus
/
Horses
/
Korea
/
Antibodies
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Journal of Bacteriology and Virology
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS