Detection of Chlamydia Trachomatis in Cultured Conjunctival Cell
Journal of the Korean Ophthalmological Society
;
: 609-613, 1991.
Article
in Korean
| WPRIM
| ID: wpr-15424
ABSTRACT
This study was performed to observe the elementary body and initial body in the cultured conjuntival epithelial cell, which was co-cultures with Chlamydia trachomatis serotype-D. Following 3 weeks of cultivation of the rabbit conjuntival epithelial cell, Chlamydia trachomatis seretype-D was inoculated into the epithelial cells and co-cultured for 24, 48, and 96 hours respectively. The infected conjunctival epithelial cells was stained with fluorescence-conjugated chlamydial antibody and iodine staining. Regardless of the duration of the cocultivation time, the cultured conjunctival cells showed the positive reaction to immunofluorescent staining and iodine staining. These results indicate that Chlamydia trachomatis can be cultured in the cultured conjuntival epothelial cell of rabbit and iodine staining is a good alternative to the immunofluorescent method.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Inclusion Bodies
/
Chlamydia
/
Chlamydia trachomatis
/
Fluorescent Antibody Technique
/
Coculture Techniques
/
Epithelial Cells
/
Iodine
Type of study:
Diagnostic study
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
1991
Type:
Article
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