Muscle Derived Stem Cell/Alginate/Polycaprolactone/ Injection Therapy in Rats with Denervated Urethral Sphincter / 대한비뇨기과학회지
Korean Journal of Urology
;
: 1296-1301, 2007.
Article
in Korean
| WPRIM
| ID: wpr-154456
ABSTRACT
PURPOSE:
In this study, we tested whether injections of muscle-derived stem cells and alginate(Alg)/polycaprolactone(PCL) after denervation of the pudendal nerve could increase the leak point pressure(LPP) and closing pressure(CP) over the long term in a rat model of urinary incontinence. MATERIALS ANDMETHODS:
Muscle derived stem cells(MDSC) were isolated from the gastrocnemius muscle of normal female rats, and these cells were purified for creating a myogenic population by the preplate technique. In the denervated(D) group, the pudendal nerve was transected bilaterally via a dorsal incision in order to denervate the external urethral sphincter. The denervated external urethral sphincter was injected with Alg/PCL(AP group), or MDSC/Alg/PCL(M group) into the proximal urethra after pudendal nerve transection. At 1 and 3 months, the LPP and CP measurements were visually identified by using the vertical tilt/intravesical pressure clamp model of stress urinary incontinence. The rats were then sacrificed and their urethras were harvested for histology.RESULTS:
Both the LPP and CP were significantly lower in the denervated group at each time compared with the normal(N group), AP and M groups, and both the LPP and CP in the N, AP and M groups were significantly higher than those in the D group at both 1 and 3 months. The persistence of MDSC over the period of the study was verified by histology. Thus, pudendal nerve denervation led to a progressive decline in the LPP and CP that was evident at 1 month and this persisted to 3 months, and injection of MDSC/Alg/PCL into the denervated rats led to a long term increase in the LPP and CP.CONCLUSIONS:
The N, AP and M groups all had significantly higher LPPs than the D group, and MDSC/Alg/PCL injection into the denervated external urethral sphincter in female rats increased the LPP and CP in both the short and long term. We also observed a long term bulking effect of MDSC/Alg/PCL injection in the stress incontinence animal model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stem Cells
/
Urethra
/
Urinary Incontinence
/
Muscle, Skeletal
/
Models, Animal
/
Denervation
/
Pudendal Nerve
Limits:
Animals
/
Female
/
Humans
Language:
Korean
Journal:
Korean Journal of Urology
Year:
2007
Type:
Article
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