Long-Term Effects of Bone Marrow-Derived Mesenchymal Stem Cells in Dextran Sulfate Sodium-Induced Murine Chronic Colitis
Gut and Liver
;
: 412-419, 2016.
Article
in English
| WPRIM
| ID: wpr-155141
ABSTRACT
BACKGROUND/AIMS:
Bone marrow-derived mesenchymal stem cells (BM-MSCs) have shown beneficial effects in experimental colitis models, but the underlying mechanisms are not fully understood. We investigated the long-term effects of BM-MSCs, particularly in mice with chronic colitis.METHODS:
Chronic colitis was induced by administering 3% dextran sulfate sodium (DSS) in a series of three cycles. BM-MSCs were injected intravenously into DSS-treated mice three times during the first cycle. On day 33, the therapeutic effects were evaluated with clinicopathologic profiles and histological scoring. Inflammatory mediators were measured with real-time polymerase chain reaction.RESULTS:
Systemic infusion of BM-MSCs ameliorated the severity of colitis, and body weight restoration was significantly promoted in the BM-MSC-treated mice. In addition, BM-MSC treatment showed a sustained beneficial effect throughout the three cycles. Microscopic examination revealed that the mice treated with BM-MSCs had fewer inflammatory infiltrates, a lesser extent of inflammation, and less crypt structure damage compared with mice with DSS-induced colitis. Anti-inflammatory cytokine levels of interleukin-10 were significantly increased in the inflamed colons of BM-MSC-treated mice compared with DSS-induced colitis mice.CONCLUSIONS:
Systemic infusion of BM-MSCs at the onset of disease exerted preventive and rapid recovery effects, with long-term immunosuppressive action in mice with repeated DSS-induced chronic colitis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Body Weight
/
Bone Marrow
/
Inflammatory Bowel Diseases
/
Dextran Sulfate
/
Dextrans
/
Interleukin-10
/
Colitis
/
Colon
/
Mesenchymal Stem Cells
/
Real-Time Polymerase Chain Reaction
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Gut and Liver
Year:
2016
Type:
Article
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