Possible Role of Heme Oxygenase-1 and Prostaglandins in the Pathogenesis of Cerebral Malaria: Heme Oxygenase-1 Induction by Prostaglandin D2 and Metabolite by a Human Astrocyte Cell Line
The Korean Journal of Parasitology
;
: 15-21, 2010.
Article
in English
| WPRIM
| ID: wpr-155206
ABSTRACT
Astrocytes are the most abundant cells in the central nervous system that play roles in maintaining the blood-brain-barrier and in neural injury, including cerebral malaria, a severe complication of Plasmodium falciparum infection. Prostaglandin (PG) D2 is abundantly produced in the brain and regulates the sleep response. Moreover, PGD2 is a potential factor derived from P. falciparum within erythrocytes. Heme oxygenase-1 (HO-1) is catalyzing enzyme in heme breakdown process to release iron, carbon monoxide, and biliverdin/bilirubin, and may influence iron supply to the P. falciparum parasites. Here, we showed that treatment of a human astrocyte cell line, CCF-STTG1, with PGD2 significantly increased the expression levels of HO-1 mRNA by RT-PCR. Western blot analysis showed that PGD2 treatment increased the level of HO-1 protein, in a dose- and time-dependent manner. Thus, PGD2 may be involved in the pathogenesis of cerebral malaria by inducing HO-1 expression in malaria patients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmodium falciparum
/
Cell Line
/
Prostaglandins
/
Blotting, Western
/
Astrocytes
/
Malaria, Falciparum
/
Malaria, Cerebral
/
Reverse Transcriptase Polymerase Chain Reaction
/
Gene Expression Profiling
/
Heme Oxygenase-1
Type of study:
Etiology study
Limits:
Animals
/
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2010
Type:
Article
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