Flow Cytometry-Assisted Basophil Activation Test as a Safe Diagnostic Tool for Aspirin/NSAID Hypersenstivity
Allergy, Asthma & Immunology Research
;
: 137-142, 2012.
Article
in English
| WPRIM
| ID: wpr-155321
ABSTRACT
PURPOSE:
Aspirin and non-steroidal anti-inflammatory drugs (ASA/NSAIDs) are common causes of drug hypersensitivity. An oral provocation test is the only definitive diagnostic test. This study assessed the reliability of a flow cytometry-assisted basophil activation test (FAST) as a safe diagnostic method for ASA/NSAID-induced hypersensitivity, as its high sensitivity and specificity have been demonstrated for many other drugs.METHODS:
Eighteen patients and 11 controls were enrolled. Using a Flow-CAST kit(R) (Buhlmann Laboratories AG, Schonenbuch, Switzerland), 29 analyses with aspirin, ibuprofen, and diclofenac were performed by flow cytometry to detect double-positive staining of anti-IgE and anti-CD63. The stimulation index was defined as the activated basophil percentage after drug stimulation/basally active basophil percentage. A stimulation index> or =2 and an absolute activated basophil percentage> or =5 were considered positive.RESULTS:
Patients with hypersensitivity to ASA/NSAIDs were predominantly female, and the prevalence of atopy was higher in patients than in controls. A sensitivity of 61%, specificity of 91%, positive predictive value of 92%, and negative predictive value of 59% were achieved.CONCLUSIONS:
FAST is a useful additional method for diagnosis of hypersensitivity reactions to ASA/NSAIDs. Further development is required to increase the sensitivity of the test.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Basophils
/
Aspirin
/
Diclofenac
/
Ibuprofen
/
Antibodies, Anti-Idiotypic
/
Prevalence
/
Diagnostic Tests, Routine
/
Drug Hypersensitivity
/
Flow Cytometry
/
Hypersensitivity
Type of study:
Diagnostic study
/
Prevalence study
Limits:
Female
/
Humans
Language:
English
Journal:
Allergy, Asthma & Immunology Research
Year:
2012
Type:
Article
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