Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR
The Korean Journal of Parasitology
;
: 573-577, 2013.
Article
in English
| WPRIM
| ID: wpr-155353
ABSTRACT
A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Swine Diseases
/
Azure Stains
/
Toxoplasma
/
Biological Assay
/
Brain
/
Toxoplasmosis, Animal
/
DNA, Protozoan
/
Sensitivity and Specificity
/
Parasitemia
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2013
Type:
Article
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