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Lentivirus-mediated RNA interference targeting E2F-1 inhibits human gastric cancer MGC-803 cell growth in vivo
Experimental & Molecular Medicine ; : 638-645, 2011.
Article in English | WPRIM | ID: wpr-155752
ABSTRACT
The E2F-1 transcription factor is post-translationally modified and stabilized in response to various forms of DNA damage to regulate the expression of cell-cycle and pro-apoptotic genes. The sustained overexpression of E2F-1 is a characteristic feature of gastric cancer. In this study, we investigated the role of short hairpin RNA (shRNA) targeting E2F-1 gene on human gastric cancer MGC-803 cell growth in vivo, and preliminarily revealed the mechanism. Thus, we constructed recombinant pGCSIL-GFP-shRNA-E2F-1 lentiviral vector to knock down E2F-1 expression in human gastric cancer MGC-803 cells in vivo, and studied the effect of E2F-1 shRNA on growth of MGC-803 tumor and evaluated its treatment efficacy. Our data demonstrated that in a mouse model of established gastric cancer, intratumor injection of lentiviral shRNA targeting E2F-1 definitely decreased the endogenous E2F-1 mRNA and protein expression in MGC-803 tumor, and inhibited tumor growth and promoted tumor cells apoptosis. Moreover, we found that E2F-1 shRNA increased the expression of phosphatase and tensin homolog (PTEN), activated caspase-3 and caspase-9, and suppressed nuclear factor (NF)-kappaB expression in tumor tissue as determined by reverse transcription (RT)-PCR and western blotting. In summary, shRNA targeting of E2F-1 can effectively inhibits human gastric cancer MGC-803 cell growth in vivo and may be a potential therapeutic strategy for gastric cancer.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Stomach Neoplasms / Cells, Cultured / Blotting, Western / Apoptosis / Lentivirus / RNA, Small Interfering / RNA Interference / E2F1 Transcription Factor / Caspase 3 / Caspase 9 Type of study: Prognostic study Limits: Animals / Humans / Male Language: English Journal: Experimental & Molecular Medicine Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Stomach Neoplasms / Cells, Cultured / Blotting, Western / Apoptosis / Lentivirus / RNA, Small Interfering / RNA Interference / E2F1 Transcription Factor / Caspase 3 / Caspase 9 Type of study: Prognostic study Limits: Animals / Humans / Male Language: English Journal: Experimental & Molecular Medicine Year: 2011 Type: Article