Tumor Necrosis Factor-alpha Gene Polymorphism (C-850T) in Korean Patients with Preeclampsia
Journal of Genetic Medicine
;
: 155-160, 2009.
Article
in English
| WPRIM
| ID: wpr-15585
ABSTRACT
PURPOSE:
Preeclampsia is a multisystem human pregnancy-specific disorder. The pathophysiology of preeclampsia is linked with over-stimulation of inflammatory cytokines by placental ischemia via reduced uterine perfusion pressure during pregnancy. Although an increase in tumor necrosis factor (TNF)-alpha has been reported in preeclamptic women, there is little evidence of a relationship between TNF-alpha gene variations and preeclampsia. In this study, we identified a single-nucleotide polymorphism (SNP), C-850T, in the TNF-alpha gene promoter region in Korean preeclamptic women and investigated the association between this SNP and the development of preeclampsia. MATERIALS ANDMETHODS:
This polymorphism was analyzed in peripheral blood samples from 198 preeclamptic pregnancies and 194 normotensive pregnancies using a SNapShot kit and an ABI Prism 3100 Genetic analyzer.RESULTS:
Genotypes and allele frequencies for C-850T did not differ between preeclamptic and normotensive pregnancies. The distributions of genotypes (CC, CT and TT) were 74.3%, 22.2% and 3.5%, respectively, in preeclamptic pregnancies, and 71.6%, 25.8% and 2.6%, respectively, in normotensive pregnancies. The frequencies of the C and T alleles were 0.85 and 0.15 in preeclamptic pregnancies and 0.84 and 0.16 in normotensive pregnancies, respectively. There was no increased risk of preeclampsia in subjects with the CT (OR, 0.83; P=0.44) or TT genotypes (OR, 1.32; P=0.64).CONCLUSION:
We found no differences in the genotypes or allele frequencies of the TNF-alpha gene polymorphism between preeclamptic and normotensive pregnancies. This study suggests that the TNF-alpha gene polymorphism may be not associated with the development of preeclampsia in pregnant Korean women.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Perfusion
/
Pre-Eclampsia
/
Cytokines
/
Promoter Regions, Genetic
/
Tumor Necrosis Factor-alpha
/
Alleles
/
Gene Frequency
/
Genotype
/
Ischemia
Type of study:
Prognostic study
Limits:
Female
/
Humans
/
Pregnancy
Language:
English
Journal:
Journal of Genetic Medicine
Year:
2009
Type:
Article
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