Investigation of Oxidative Stress and Development of Lower Urinary Tract Symptoms in the Urinary Bladder following Partial Bladder Outlet Obstruction by Proteomic Approach / 대한비뇨기과학회지

ABSTRACT

PURPOSE: This study investigated the pathophysiological mechanism using the proteomic approach to detect the marker proteins for the development of lower urinary tract symptoms following a partial bladder outlet obstruction (BOO). MATERIALS AND METHODS: Rats were randomized into 3 groups, the control, sham operation and BOO groups. The BOO group was divided into 1, 3, and 5 day-groups. Conventional proteomics was performed using high resolution 2-D gel electrophoresis followed by computational image analysis and protein identification using mass spectrometry using the rat urinary bladders. RESULTS: A comparison of the bladder from the BOO group with that from the sham control bladder showed three proteins, optineurin (OPTN), thioredoxin and preprohaptoglobin, to be over-expressed in the bladder of the BOO group. In addition, four proteins; peroxiredoxin 2, transgelin, hippocampal cholinergic neurostimulating peptide (HCNP) and beta-galactoside-binding lectin were under-expressed in the bladder of the BOO group. CONCLUSIONS: The down-regulation of HCNP might make the detrusor muscle supersensitive to acetylcholine, and the up-regulation of OPTN indicates protection from nerve injury. In addition, the up-regulation of thioredoxin and preprohaptoglobin and the down-regulation of peroxiredoxin 2 indicate that BOO may be related to oxidative stress. However more information on human bladder tissue will be needed for clinical usage and a urodynamic study.