Investigation of Oxidative Stress and Development of Lower Urinary Tract Symptoms in the Urinary Bladder following Partial Bladder Outlet Obstruction by Proteomic Approach / 대한비뇨기과학회지
Korean Journal of Urology
;
: 1337-1343, 2005.
Article
in Korean
| WPRIM
| ID: wpr-156530
ABSTRACT
PURPOSE:
This study investigated the pathophysiological mechanism using the proteomic approach to detect the marker proteins for the development of lower urinary tract symptoms following a partial bladder outlet obstruction (BOO). MATERIALS ANDMETHODS:
Rats were randomized into 3 groups, the control, sham operation and BOO groups. The BOO group was divided into 1, 3, and 5 day-groups. Conventional proteomics was performed using high resolution 2-D gel electrophoresis followed by computational image analysis and protein identification using mass spectrometry using the rat urinary bladders.RESULTS:
A comparison of the bladder from the BOO group with that from the sham control bladder showed three proteins, optineurin (OPTN), thioredoxin and preprohaptoglobin, to be over-expressed in the bladder of the BOO group. In addition, four proteins; peroxiredoxin 2, transgelin, hippocampal cholinergic neurostimulating peptide (HCNP) and beta-galactoside-binding lectin were under-expressed in the bladder of the BOO group.CONCLUSIONS:
The down-regulation of HCNP might make the detrusor muscle supersensitive to acetylcholine, and the up-regulation of OPTN indicates protection from nerve injury. In addition, the up-regulation of thioredoxin and preprohaptoglobin and the down-regulation of peroxiredoxin 2 indicate that BOO may be related to oxidative stress. However more information on human bladder tissue will be needed for clinical usage and a urodynamic study.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Mass Spectrometry
/
Thioredoxins
/
Urodynamics
/
Urinary Bladder
/
Urinary Bladder Neck Obstruction
/
Electrophoresis, Gel, Two-Dimensional
/
Acetylcholine
/
Down-Regulation
/
Up-Regulation
/
Oxidative Stress
Type of study:
Controlled clinical trial
/
Diagnostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Korean Journal of Urology
Year:
2005
Type:
Article
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