Fresh Fibroblast Allograft as a Treatment for Diabetic Foot Ulcers
Journal of the Korean Society of Plastic and Reconstructive Surgeons
;
: 501-506, 2008.
Article
in Korean
| WPRIM
| ID: wpr-156603
ABSTRACT
PURPOSE:
In order to overcome the limitations of the conventional cryopreserved fibroblast or keratinocyte allograft method used in the treatment of diabetic foot ulcers, we reported a pilot study in 2004 demonstrating promising results of a fresh fibroblast allograft method in eight patients. However, the number of cases was insufficient for full evaluation and the follow-up duration was not long enough to determine the efficacy and safety of the method. This encouraged us to conduct this follow-up study to fully evaluate the use of noncryopreserved fresh human fibroblast allografts in treating diabetic foot ulcers.METHODS:
Thirty-seven patients with diabetic foot ulcers were treated using fresh fibroblast allografts. Human dermal fibroblasts from healthy teenagers were cultured in DMEM/F-12 medium supplemented with 10% serum. The cultured cells were applied on the wounds immediately following debridement, with fibrin being used as a cell carrier. In eight weeks, percentages of complete healing, mean healing time, and patient satisfactions were assessed, with follow-up time ranging from 6 to 40 months.RESULTS:
Our study showed that 83.8% of the treated patients were complete healed. The time required for complete healing was 30.9+/-10.1 days. Patient satisfaction scores for the experimental treatment were higher than those for the conventional method(mean scores of 8.1+/-1.1 and 4.8+/-1.4, respectively). No adverse events related to the study treatment occurred.CONCLUSION:
The use of fresh human fibroblast allografts was found to be a safe and effective treatment for diabetic foot ulcers.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transplantation, Homologous
/
Ulcer
/
Fibrin
/
Keratinocytes
/
Cells, Cultured
/
Pilot Projects
/
Follow-Up Studies
/
Patient Satisfaction
/
Diabetic Foot
/
Debridement
Type of study:
Observational study
/
Prognostic study
Limits:
Adolescent
/
Humans
Language:
Korean
Journal:
Journal of the Korean Society of Plastic and Reconstructive Surgeons
Year:
2008
Type:
Article
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