Nationwide Survey of Stool Culture Methods for the Diagnosis of Bacterial Gastroenteritis in Korea, 2016
Journal of Laboratory Medicine and Quality Assurance
;
: 23-30, 2017.
Article
in Korean
| WPRIM
| ID: wpr-156757
ABSTRACT
BACKGROUND:
Stool cultures are essential for diagnosing bacterial gastrointestinal infections. Laboratory procedures and target organisms for stool culture testing can vary by institute. Therefore, a nationwide survey was conducted to determine the stool culture procedures performed in clinical laboratories of Korea.METHODS:
Questionnaires were delivered by electronic mail to 98 clinical microbiologists and by Google survey to the 301 institutes participating in the Korean External Quality Control Program of Bacterial Cultures.RESULTS:
Of the 68 institutes sent complete responses, Gram staining and wet smears were performed in 73.5% and 64.7%, respectively. A molecular test was conducted in 32.4% of laboratories, and blood agar plates were used in 23.5%. Staphylococcus aureus , Pseudomonas aeruginosa , and Candida species were reported for predominant growth by 17.6%, 8.8%, and 7.4% of the respondents, respectively. Campylobacter culture was available only in 25.0% of laboratories, whereas Clostridium difficile could be cultivated in 38.2%. Susceptibility testing results of Salmonella-Shigella were reported for all tested antibiotics in 22.1% of laboratories, whereas 69.1% reported results for antibiotics specified by the Clinical and Laboratory Standard Institute guidelines.CONCLUSIONS:
Methods and results of gram staining, wet smears, use of stool culture media, target microorganisms, and antibiotic susceptibility differed among the institutes. Further discussion is needed to develop a standardized protocol for stool culture to maximize isolation of bacterial pathogens that cause gastroenteritis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pseudomonas aeruginosa
/
Quality Control
/
Staphylococcus aureus
/
Campylobacter
/
Candida
/
Surveys and Questionnaires
/
Clostridioides difficile
/
Culture Media
/
Agar
/
Electronic Mail
Type of study:
Diagnostic study
/
Practice guideline
/
Qualitative research
Country/Region as subject:
Asia
Language:
Korean
Journal:
Journal of Laboratory Medicine and Quality Assurance
Year:
2017
Type:
Article
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